Cells of peripheral (whole) blood comprises of immune cells including [B-cells, T-cells,
Dendritic cells, Monocytes, NK cells] = PBMCs, Basophils, Eosinophils, Granulocytes
and Neutrophils. Non-immune cells are also present such as Red blood cells and Platelets.
Standard procedures are employed to purify or enrich for these cells. For example,
PBMCs is isolated by centrifugation of blood in ficoll.
Flow cytometry (FCM) is an analytical tool that is used regularly to determine the
percentage of cells in blood and their subsets (e.g., CD4+ T-cells) and investigate
markers present on the surface or inside (intracellular) the cells. Moreover, gene
expression and the overall state of the cell can be assessed by FCM.
As with any experiment, FCM experiments require the use of controls. These controls
address questions such as the integrity of the detecting antibody and the specificity
of the antibody. PBMCs are used as controls to answer these and other questions allowing
the investigator to quickly evaluate cell staining results conclusively with clarity
and confidence. Cells Depot™ Preserved human PBMCs. Compatible with antibodies from
major manufacturers (e.g., BD Biosciences, BioLegend, eBioscience and Tonbo BioSciences).
Human Peripheral Blood Mononuclear Cells (PBMC) for FCM