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PBMCs for Flow Cytometry | go to product page |


Cells of peripheral (whole) blood comprises of immune cells including [B-cells, T-cells, Dendritic cells, Monocytes, NK cells] = PBMCs, Basophils, Eosinophils, Granulocytes and Neutrophils. Non-immune cells are also present such as Red blood cells and Platelets. Standard procedures are employed to purify or enrich for these cells. For example, PBMCs is isolated by centrifugation of blood in ficoll.


Flow cytometry (FCM) is an analytical tool that is used regularly to determine the percentage of cells in blood and their subsets (e.g., CD4+ T-cells) and investigate markers present on the surface or inside (intracellular) the cells. Moreover, gene expression and the overall state of the cell can be assessed by FCM.


As with any experiment, FCM experiments require the use of controls. These controls address questions such as the integrity of the detecting antibody and the specificity of the antibody. PBMCs are used as controls to answer these and other questions allowing the investigator to quickly evaluate cell staining results conclusively with clarity and confidence. Cells Depot™ Preserved human PBMCs. Compatible with antibodies from major manufacturers (e.g., BD Biosciences, BioLegend, eBioscience and Tonbo BioSciences).

Human Peripheral Blood Mononuclear Cells (PBMC) for FCM